Show Summary Details
Page of

Optogenetics and Electrophysiology 

Optogenetics and Electrophysiology
Optogenetics and Electrophysiology

Hua-an Tseng

, Richie E. Kohman

, and Xue Han

Page of

PRINTED FROM OXFORD MEDICINE ONLINE ( © Oxford University Press, 2021. All Rights Reserved. Under the terms of the licence agreement, an individual user may print out a PDF of a single chapter of a title in Oxford Medicine Online for personal use (for details see Privacy Policy and Legal Notice).

date: 28 July 2021

Throughout the history of neuroscience, electrophysiological and imaging techniques have been utilized to observe neural signals at various spatial and temporal scales. However, it has been difficult to manipulate the activity of specific cells or neural circuits with the spatial and temporal resolutions relevant to neural coding. A novel technique called optogenetics, has recently been developed to control the activity of specific cells. This technique allows rapid and reversible optical activation or silencing of specific cells, which have been genetically transduced with light-sensitive molecules. The development of microbial opsin-based optogenetic molecular sensors has made optogenetics easily adaptable in various in vivo and in vitro preparations, and the technique has already been applied to understand neural circuit mechanisms of many behaviors and diseases. Here, we provide an introduction to optogenetics, the practical concerns in using the technique in vivo, and examples of applications that combine traditional electrophysiology techniques with optogenetics.

Access to the complete content on Oxford Medicine Online requires a subscription or purchase. Public users are able to search the site and view the abstracts for each book and chapter without a subscription.

Please subscribe or login to access full text content.

If you have purchased a print title that contains an access token, please see the token for information about how to register your code.

For questions on access or troubleshooting, please check our FAQs, and if you can't find the answer there, please contact us.