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Patch Clamp Recording in Brain Slices 

Patch Clamp Recording in Brain Slices
Chapter:
Patch Clamp Recording in Brain Slices
Author(s):

Luke Campagnola

and Paul Manis

DOI:
10.1093/med/9780199939800.003.0001
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date: 05 December 2020

Patch clamp recording in brain slices allows unparalleled access to neuronal membrane signals in a system that approximates the in-vivo neural substrate while affording greater control of experimental conditions. In this chapter we discuss the theory, methodology, and practical considerations of such experiments including the initial setup, techniques for preparing and handling viable brain slices, and patching and recording signals. A number of practical and technical issues faced by electrophysiologists are also considered, including maintaining slice viability, visualizing and identifying healthy cells, acquiring reliable patch seals, amplifier compensation features, hardware configuration, sources of electrical noise and table vibration, as well as basic data analysis issues and some troubleshooting tips.

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